《植物生理学报》 2010, 46(5): 448-452

非生物胁迫下水稻OsLRR 基因的表达分析

张建军¹, 胥华伟², 谭锦汶¹, 陈建东¹, 王玉琪^1,*, 彭新湘¹

1 华南农业大学生命科学学院分子植物生理研究室, 广州510642; 2 河南科技大学农学院, 河南洛阳471003

通信作者：王玉琪；E-mail: wyq@scau.edu.cn；Tel: 020-85280194

摘　要：

植物中含有多种富含亮氨酸重复(leucine-rich repeats, LRRs)的蛋白质, 这类蛋白质在植物生长、发育和抗病反应等方 面发挥着重要作用。本研究在水稻中克隆到一个编码LRRs 结构的基因OsLRR, 以半定量 RT-PCR 检测了OsLRR 在水稻不 同组织和不同非生物胁迫的表达情况, 并进一步分析了铝毒胁迫下 OsLRR 在抗铝和铝敏感水稻品种之间的表达差异。结 果表明 OsLRR 在水稻根、叶鞘和叶中都有较高表达。铝、砷、PEG6000 和 ABA 可诱导水稻根中 OsLRR 的表达, 而镉、硝 普钠和铁则抑制其表达。只有盐胁迫能诱导叶片中OsLRR 的表达。铝毒可以诱导抗铝和铝敏感水稻品种根中OsLRR 的表 达, 但随着处理时间的延长, 抗铝品种中OsLRR 的表达逐渐加强, 而铝敏感品种中OsLRR 的表达则逐渐减弱。

关键词：水稻; OsLRR; 半定量RT-PCR; 非生物胁迫

收稿：2010-01-22   修定：2010-03-08

资助：国家自然科学基金(30700052)、广东省自然科学基金博士启动项目(4600-E09022)和河南科技大学博士启动基金(09001271)。

Expression Analysis of OsLRR in Rice under Abiotic Stresses

ZHANG Jian-Jun¹, XU Hua-Wei², TAN Jin-Wen¹, CHEN Jian-Dong¹, WANG Yu-Qi^1,*, PENG Xin-Xiang¹

¹Laboratory of Molecular Plant Physiology, College of Life Sciences, South China Agricultural University, Guangzhou 510642, China; ²College of Agriculture, Henan University of Science and Technology, Luoyang, Henan 471003, China

Corresponding author: WANG Yu-Qi; E-mail: wyq@scau.edu.cn; Tel: 020-85280194

Abstract:

There are many proteins with leucine-rich repeats (LRRs) in plants, which play important roles in plant growth, development and disease resistance. In this study, a gene in rice, OsLRR, which encodes a protein with leucine rich repeats, was cloned by PCR. The expression patterns of OsLRR in different tissues or under various abiotic stresses were analyzed by semi-quantitative RT-PCR, and the difference between an aluminum (Al)-resistant cultivar and an Al-susceptible cultivar under Al stress was also observed. The results showed that the OsLRR was highly expressed in roots, leaf sheahs and leaves. The expression level of OsLRR was highly induced by Al, arsenic, PEG6000 and ABA, while inhibited by cadmium, SNP (sodium nitroprusside) and iron toxicity in rice roots. In rice leaves, OsLRR could only be induced by salt stress. In addition, its expression in rice roots was gradually enhanced in the Al-resistant cultivar while reduced in the Al-susceptible cultivar under Al stress.

Key words: rice; OsLRR; semi-quantitive RT-PCR; abiotic stresses